Summary
Type: SPE
Application #: 304550
Catalog#: 730180 - SPE Cartridges, Chromabond, NH2, 6ml, PP, 500mg 30/pk
Detection: 50 µL of the elution were injected into the HPLC system without further concentration. HPLC analysis was performed using an C18 column (e.g. EC 150/4.6NUCLEODUR® 100-5 C18ec; Cat.-No. 760008.46) by is
Conditions: Column conditioning:
5mL of a solution containing 0.05 mol/L Na2HPO4 solution of pH 9.0, methanol and acetonitrile (60:20:20), using a flow rate of 1mL/min
Sample aspiration:
7 mL (coffee samples) respectively 10 mL (cheese samples) of the resulting solutions were passed through the SPE cartridge using a flow rate of 1mL/min
Column washing / Elution:
(no separate washing or elution steps are necessary – carbolines will pass the column without retention – matrix -compounds will be removed)
Authors: SPE department
Source: Macherey-Nagel